Open Biological Sciences Journal
2018, 4 : 1-6Published online 2018 February 28. DOI: 10.2174/2352633501804010001
Publisher ID: BIOLSCI-4-1
RESEARCH ARTICLE
Antifungal Susceptibility Pattern of Candida Albicans in Human Infections
* Address correspondence to this author at the Biotechnology Pharmacology Laboratory and Human genetics Laboratory,Centre for Scientific Research and Development, People’s University, Bhopal 462037, India; Tel: 02554004084; E-mails: , abinmani@gmail.com
ABSTRACT
Introduction:
Candida albicans is an opportunistic pathogen under immune-compromised conditions and despite anti-fungal therapies, it has become lethal. Increase in the antimicrobial resistance in C. albicans is a matter of concern since it is in the human microbiome.
Aims and Objectives:
This study was conducted to estimate the incidence of C. albicans in infections, and evaluate its antifungal susceptibility in clinical samples.
Methods and Materials:
Two hundred isolates of C. albicans from different clinical samples were analyzed against its susceptibility towards four antifungal agents (fluconazole, ketoconazole, itraconazole and amphotericin-B) using well diffusion and MIC by microdilution assay.
Results:
All isolates in the study were sensitive to amphotericin-B and ketoconazole and a high frequency of fluconazole and itraconazole resistance was observed. Oral and catheter tips were observed to be the major sites of C. albicans infections. Significant resistance aganist fluconazole (56.5%) and itraconazole (64.5%) was observed with MIC at 16, 32 to 64μg/ml. All isolates were observed to be sensitive for ketoconazole and amphotericin-B at 0.5μg/ml and 1μg/ml.
Conclusion:
The study shows a higher antibiotic resistance in the clinical samples which proves the risk in C. albicans management program.