Current Chemical Genomics and Translational Medicine
2011, 5 : 122-128Published online 2011 November 30. DOI: 10.2174/1875397301105010122
Publisher ID: CCGTM-5-122
RESEARCH ARTICLE
A Cell-Based Protein-Protein Interaction Method Using a Permuted Luciferase Reporter
1
BioInvenu Corporation, USA, East Hanover, NJ
2 Sanofi R&D, Bridgewater, NJ
3 Sanofi R&D, Oro Valley, AZ, USA
* Address correspondence to this author at the BioInvenu Corporation, USA, East Hanover, NJ, USA; Tel: 973-585-6777; Fax: 973-585-6776; E-mail: haifeng.eishingdrelo@bioinvenu.com
2 Sanofi R&D, Bridgewater, NJ
3 Sanofi R&D, Oro Valley, AZ, USA
* Address correspondence to this author at the BioInvenu Corporation, USA, East Hanover, NJ, USA; Tel: 973-585-6777; Fax: 973-585-6776; E-mail: haifeng.eishingdrelo@bioinvenu.com
ABSTRACT
We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another protein (B) fused with the TEV protease. We demonstrate assay applicability for ligand-induced protein-protein interactions including G-protein coupled receptors, receptor tyrosine kinases and nuclear hormone receptors.