RESEARCH ARTICLE
Synthesis, Characterization and Antimicrobial Evaluation of Novel Mannich Bases Containing Pyrazole-5-One Phosphonates

Newly synthesised compounds of phosphonates were prepared by condensation of diethylphosphate with imine which undergoes a reaction of mannich bases with pyrazole containing schiffs base. The base was prepared by condensation of aldehyde with primary amine. These newly synthesised derivatives were characterised by spectral analysis.

Mannich bases are very important to synthesize wide variety of natural products and pharmaceuticals.

Thin Layer Chromatography was performed on aluminum sheet of silica gel 60F254, E-Merk, Germany using iodine as visualizing agent. IR Spectra were recorded as KBr pellets on Perkin-Elmer 1000 units, instruments. All ¹H and ¹³C-NMR spectra were recorded on a Varian XL-300 spectrometer operating at 400MHz and 75 MHz. ³¹P-NMR spectra were recorded on a Varian XL-spectrometer operating at 161.89MHz. The compounds were dissolved in dimethylsulfoxide and Chemical shifts were referenced to Trimethylsilane (¹H and ¹³C-NMR) and 85% phosphoric acid (³¹P-NMR).

Some of the novel synthetic compounds of Pyrazole Mannich base-Phosphonates showed great potential in field of medicinal chemistry and good biological activity.

It can be concluded that this class of compounds certainly holds great potential for the discovery of novel classes of antimicrobial agents.