The Open Analytical Chemistry Journal
2007, 1 : 28-37Published online 2007 December 12. DOI: 10.2174/1874065000701010028
Publisher ID: TOACJ-1-28
Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge
Division of Pharmaceutical
and Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy
and Life Science 1432-1 Horinouchi, Hachioji, Tokyo 192-0392,
Japan.
ABSTRACT
This review describes protein purifications from cell culture medium or cell-lysate by high speed countercurrent chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.