In Silico Evidence of the Relationship Between miRNAs and siRNAs

ABSTRACT

Both short interfering RNAs (siRNAs) and microRNAs (miRNAs) mediate the repression of specific sequences of mRNA through the RNA interference pathway. In the last years several experiments have supported the hypothesis that siRNAs and miRNAs may be functionally interchangeable, at least in cultured cells. In this work we verify that this hypothesis is also supported by a computational evidence. We show that a method specifically trained to predict the activity of the exogenous siRNAs assigns a high silencing level to experimentally determined human miRNAs. This result not only supports the idea of the biological interchangeability of siRNAs and miRNAs but indicates that it is possible to use computational tools developed using synthetic small interference RNAs to study endogenous miRNAs.