CD4CD25 Regulatory T Cells Preventing Detrimental Autoimmune Reactions

ABSTRACT

Autoimmune diseases will occur when the immune response inflicts damage to tissues in the body. Attempts to overcome this disease have a lot to do but they have not yielded satisfying results. In this study, the function of CD4⁺CD25⁺ regulatory T cells was evaluated for therapeutic potential to prevent the development of autoreactive T cells.

The isolation of CD4⁺CD25⁺ regulatory T cells was performed by using FACS vantage. It was conducted by taking a spleen of 6-week-old C57BL/6 mice. Cells having high purity (2x106) were intravenously injected into 3-weekold CD122^-/- mice. In this experiment we used congeneic mice as donors and transferred hosts. The result of adoptive transfer was examined after 7 weeks.

Here we showed that the transfer of CD4⁺CD25⁺ regulatory T cells into CD122^-/- mice significantly prevented the development of abnormal T cells. The injection of CD4⁺CD25⁺ regulatory T cells derived from normal young mice into CD122^-/- mice could prevent the development of activated memory T cells and these cells were essential for maintaining normal homeostasis (P >0.01).

In this study we obtained evidence that regulatory T cells (2x10⁶) derived from normal young mice could overcome diseases when transferred to the mouse models of autoimmune disorder.

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