The Open Bioactive Compounds Journal
2008, 1 : 22-27Published online 2008 November 28. DOI: 10.2174/1874847300801010022
Publisher ID: TOBCJ-1-22
RESEARCH ARTICLE
Sphingosine Kinase 2 Inhibitor Accelerates Fas-Mediated Cell Death Progression in A20/2J Cells
1
Department of Hard Tissue Engineering, Biochemistry, Division of Bio-Matrix, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo 113-8549, Japan,
2 Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12-jo, Nishi 6-choume, Kita-ku, Sapporo 060-0812, Japan,
3 LCS biotech, 3-26, Pil-dong, Chung-gu, Seoul 100-715, Korea, (Present address)
4 Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul 100-715, Korea (Present address)
5 Brain Science Institute, The Institute of Physical and Chemical Research (Riken), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
* Address correspondence to this author at the Department of Hard Tissue Engineering, Biochemistry, Division of Bio-Matrix, Graduate School Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo 113-8549, Japan; Tel: +81 3 5803 5567; Fax: +81 3 5803 0187; E-mail: m.yokoyama.bch@tmd.ac.jp
2 Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12-jo, Nishi 6-choume, Kita-ku, Sapporo 060-0812, Japan,
3 LCS biotech, 3-26, Pil-dong, Chung-gu, Seoul 100-715, Korea, (Present address)
4 Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul 100-715, Korea (Present address)
5 Brain Science Institute, The Institute of Physical and Chemical Research (Riken), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
* Address correspondence to this author at the Department of Hard Tissue Engineering, Biochemistry, Division of Bio-Matrix, Graduate School Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo 113-8549, Japan; Tel: +81 3 5803 5567; Fax: +81 3 5803 0187; E-mail: m.yokoyama.bch@tmd.ac.jp
ABSTRACT
A sphingosine kinase (SPHK) type 2 inhibitor, SG-12, inhibited the SPHK2 activity in A20/2J cells and promoted Fas-dependent cell death. The effect of SG-12 on cell death was relieved by SPHK2 transfection but not by SPHK1 transfection. The results suggest a SPHK2-specific suppression of Fas-dependent cell death.