The Open Bioactive Compounds Journal
2013, 4 : 4-13Published online 2013 September 6. DOI: 10.2174/1874847320130701002
Publisher ID: TOBCJ-4-4
RESEARCH ARTICLE
Effect of resveratrol and its hydroxylated analogues on proliferation and apoptosis of
two cervix cancer derived cancer cell lines. The role of mitochondrial superoxide
dismutase
2 Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, Poznan, Poland
3 Department of Cancer Immunology, Poznan University of Medical Sciences at GreatPoland Cancer Center, Poland
* Address correspondence to this author at the Department of Toxicology, Poznan University of Medical Sciences, ul. Dojazd 30, 60-631 Poznań, Poland; Tel: +48 61 8472081; Fax: +48 61 8470721; E-mail: marek.murias@ump.edu.pl
ABSTRACT
The naturally occurring polyphenol resveratrol (3,4',5-trihydroxy-stilbene, 3,4',5-THS, RES) has been shown as a chemopreventive and proapototic agent. Resveratrol is extensively metabolized by CYP450 enzymes. The monohydroxylation of resveratrol is catalyzed by CYP1B1 to form 3,3',4',5-THS (piceatannol), a metabolite with higher anticancer activity and stronger antioxidant properties. It was hypothesized that RES analogues (HHRAs) possessing more than 3 hydroxyl groups may act stronger against cancer cells than RES due to reactive oxygen species formed in redox-cycling reactions. In order to investigate a structure-activity relationship between pro/antioxidant properties and cytotoxicity, the HHRAs with at least 2 phenolic groups in neighborhood- 3,4,4',5-HS and 3,3',4,4',5,5'-HS were synthesized. In the present study we tested this hypothesis in a cell culture model using HeLa and C33A cancer cell lines. The results of our experiments support a hypothesis that MnSOD overexpressing HeLa cells are much more resistant to superoxide generating HHRAs than C33A cells.