The Open Bioactive Compounds Journal
2017, 5 : 23-30Published online 2017 September 26. DOI: 10.2174/1874847301705010023
Publisher ID: TOBCJ-5-23
RESEARCH ARTICLE
Marine Sp. VITMK1 Derived Pyrrolo [1, 2-A] Pyrazine-1, 4-Dione, Hexahydro-3-(2-Methylpropyl) and Its Free Radical Scavenging Activity
* Address correspondence to this author at the VIT University, Department of Biomedical Sciences, Vellore, Tamil Nadu, India; Tel: +91-416-2202477; E-mail: kkb.biomol@gmail.com
ABSTRACT
Background:
Free radical generation has been proved to be responsible for various cellular diseases. It is necessary to combat free radicals using antioxidants derived from natural sources.
Objective:
The objective of this study is to evaluate the antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp. VITMK1 isolated from mangrove sediment soil collected from Pichavaram, Tamil Nadu, India.
Methods:
The antioxidant potential of pyrrolo [1, 2-A] pyrazine-1, 4-dione, hexahydro-3-(2-methylpropyl) (diketopiperazine) extracted from Streptomyces sp. VITMK1 was studied using reducing power assay. The scavenging of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and nitric oxide (NO) radical by the compound was also studied. The cytotoxic activity of the compound on RAW 264.7 macrophage cell line was studied using MTT cell viability assay.
Results:
The compound exhibited strong DPPH radical scavenging activity (72.48±0.32% at 500 µg/mL) and NO radical scavenging activity (73.03±1.02% at 500 µg/mL). MTT cell viability assay revealed that the compound exhibited concentration-dependent cell viability and was observed to be 92% at 125 µg/mL concentration.
Conclusion:
The antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp. VITMK1 can be probed further to establish its radical scavenging activity.