The Open Bioactive Compounds Journal
2017, 05 : 31-42Published online 2017 October 31. DOI: 10.2174/1874847301705010031
Publisher ID: TOBCJ-5-31
RESEARCH ARTICLE
Antidiabetic and Antioxidant Potential of GancidinW from VITALK03
* Address correspondence to this author at the School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India; Tel: 0416-2202024; E-mail: kkb@vit.ac.in
ABSTRACT
Background:
The aim of the present study was to analyse the antidiabetic and antioxidant potential of GancidinW (GW) extracted from Streptomyces paradoxus VITALK03.
Materials and Methods:
Antidiabetic potential of GW was evaluated by assay of α-amylase and α-glucosidase inhibitory activity; haemoglobin glycosylation and yeast glucose uptake. The antioxidant potential of GW was assessed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization assay and superoxide assay. The inhibition of α-amylase and α-glucosidase by GW was also studied by in Silico molecular docking analysis.
Results:
GW (1mg/ml) showed 69.32% of α-amylase and 54.04% of α-glucosidase inhibitory activity. GW (1mg/ml) prevented haemoglobin glycosylation up to 30.92% and the glucose uptake by yeast cells was increased up to 64.38%. The binding interaction GW with α-amylase showed the least free binding energy of -6.09Kcal/mol and -7.53Kcal/mol with α-glucosidase by docking studies. GW also demonstrated moderate antioxidant activity in all the antioxidant assays performed.
Conclusion:
The results of this study suggests that the antidiabetic and antioxidant potential of GW can be probed further to develop GW as effective antidiabetic agent.