The Open Bioactive Compounds Journal

2019, 7 : 8-13
Published online 2019 December 05. DOI: 10.2174/1874847301907010008
Publisher ID: TOBCJ-7-8

RESEARCH ARTICLE
Analysis of Raspberry Ketone in Nutraceutical Formulation Using Fourier Transform Infrared Spectrophotometric Method

Hassan Y. Aboul-Enein1, * , Oana Mihaela Antochi2,3 , Gheorghe Nechifor3 and Andrei A. Bunaciu4

* Address correspondence to this author at the Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, Dokki, Cairo 12622, Egypt; Tel: +00201003678948; Fax: +0020233370931; E-mail: haboulenein@yahoo.com

ABSTRACT

Aims:

A Fourier Transform Infrared (FT-IR) spectrometric method was developed for the rapid, direct measurement of Raspberry Ketone (RK) and Caffeine (CAF) in a nutraceutical formulation.

Methods:

Conventional KBr-spectra and KBr+0.5 mg Microcrystalline Cellulose (MCC)-spectra were used as the basis for a better determination of active substances in the nutraceutical formulation. A calibration model was developed using caffeine and raspberry ketone standards of varying concentrations in the mid-infrared region (4000-400 cm-1). The Beer-Lambert law was used in data processing.

Results:

The results indicate that FT-IR spectrometry is applicable to the analytical quantification of RK and CAF in the nutraceutical formulation.

Conclusion:

The method proposed is simple, precise and not time-consuming compared to the chromatographic methods that are cited in the literature. Quantification is performed in about 10-15 minutes, including sample preparation and spectral acquisition.

Keywords:

Microcrystalline cellulose, Data processing, Ketone standards, Spectrometric method, Nutraceutical formulation, Analytical quantification.