The Open Chemical and Biomedical Methods Journal

2009, 2 : 13-17
Published online 2009 January 27. DOI: 10.2174/1875038900902010013
Publisher ID: TOCBMJ-2-13

Development of Membrane Filter with Oligosaccharide Immobilized by Click Chemistry for Influenza Virus Adsorption

Tomohisa Kato , Atsushi Miyagawa , Maria Carmelita Z. Kasuya and Kenichi Hatanaka
Institute of Industrial Science, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8505, Japan;

ABSTRACT

Membranes with immobilized GM3-type oligosaccharide were prepared and influenza virus adsorption was evaluated. GM3-type oligosaccharide which has azido group in the aglycon was synthesized by saccharide primer method. The azido group was used for combination by click chemistry with alkyne which is introduced to membranes. The position of the azido group in the aglycon of GM3-type oligosaccharide was either the farthest (12-azidododecyl), or the nearest (2-azidododecyl). The azido group in the farthest position showed higher reactivity with alkyne on the membrane than the one in the nearest the GM3-type oligosaccharide. Moreover, the membrane immobilized GM3-type oligosaccharide showed adsorption ability of influenza virus.

Keywords:

GM3, click chemistry, immobilization, influenza virus..