The Open Chemical and Biomedical Methods Journal

2010, 3 : 123-127
Published online 2010 November 1. DOI: 10.2174/1875038901003010123
Publisher ID: TOCBMJ-3-123

A Spectrophotometric Method for Diosmin Determination

Zenovia Moldovan , Andrei A. Bunaciu , Mohammed A. Al-Omar and Hassan Y. Aboul-Enein
Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo 12311, Egypt.

ABSTRACT

A rapid and sensitive spectrophotometric method for the determination of diosmin (D) has been developed. The method is based on the reaction of D with 4-aminoantipyrine (AAP) in the presence of hexacyanoferrate (III) (HCF) in an alkaline medium and subsequent formation of a purple coloured product having λmax at 524.5 nm. The conditions affecting the reaction (reagents concentration, pH, order of addition of reagents, stability in time) were optimized. Under the optimum conditions, Beer’s law was obeyed in the range of 0.3 – 35 μg mL-1 D with a correlation coefficient of 0.9993. The assay limits of detection and quantitation were 0.1 and 0.3 μg mL-1, respectively. The proposed method was successfully applied to the analysis of the drug tablets formulation. The results were compared favorably with those obtained by other reported methods.

Keywords:

Flavone glycosides, diosmin, phlebotropic drugs, vitamin like substances, 4-aminoantipyrine, spectrophotometry..