Effects of Tetracycline, EDTA and Citric Acid Application on Fluorosed Dentin and Cementum Surfaces: An In Vitro Study

ABSTRACT

Fluorosis is one of the factors that may bring about mineralization changes in teeth. Routine treatment of root biomodification is commonly followed during periodontal therapy. The purpose of the present study is to compare and evaluate the root surface changes in fluorosed and nonfluorosed teeth subsequent to the application of Tetracycline, EDTA, and Citric acid. Both fluorosed and nonfluorosed teeth comprising of periodontally healthy and diseased, were included in this study. Each of them was grouped into Tetracycline Hydrochloride, EDTA and Citric acid treatment groups. Using Scanning electron microscope (SEM), the photomicrographs of dentin and cementum specimens were obtained. Results showed that the amount of smear layer removal and exposure of collagen matrix in dentin specimens were variable in different groups. The exposure of fibrillar structures on cementum specimens were seen significantly on healthy specimens as compared to diseased specimens. Thus, the root biomodification procedure brings in definite difference between fluorosed and non-fluorosed dentin and cementum specimens.

Keywords:

Root biomodification, fluorosed dentin and cementum, scanning electron microscope, tetracycline, EDTA, citric acid.