Electrochemical Biosensors for the Detection of Pesticides

ABSTRACT

Biosensors have been developed for the detection of pesticides using integrated enzymes, antibodies, cell and DNA-based biosensors. Enzymatic determination of pesticides is most often based on inhibition of the activity of selected enzymes such as cholinesterase, acid phosphatase, ascorbate oxidase, acetolactate synthase and aldehyde dehydrogenase. Enzymatic Biosensors were developed using various electrochemical signal transducers and different electrodes. Various immobilization protocols used for the formation of a biorecognition interface are also discussed: In addition, techniques of regeneration, single amplification, and miniaturization are evaluated for the development of immunosensor. Both batch and flow-injection analyses with enzyme biosensors are most intensively developed. It included that, in the future, compact, disposable and portable devices especially designed for in-field analysis with high sensitivity, selectivity; development of arrays and multiple sensors will continue another area of intensive research for biosensors.