The Open Food Science Journal

2008, 2 : 68-71
Published online 2008 June 12. DOI: 10.2174/1874256400802010068
Publisher ID: TOFSJ-2-68

Liquid Chromatographic Determination of Malvidin-3-O-Glucoside and Malvidin 3, 5-O-Diglucoside in Wine Samples by Direct Injection

A. Rodríguez-Bernaldo de Quirós , J. López-Hernández and M.A. Lage-Yusty
Analytical Chemistry, Nutrition and Bromatology Department, Pharmacy Faculty, Campus Sur s/n, University of Santiago de Compostela, 15782 Santiago de Compostela (La Coruña), Spain.

ABSTRACT

A method for the determination of malvidin 3-O-glucoside (Oenin), and malvidin 3, 5-O-diglucoside (Malvin) in wines by on line HPLC coupled with UV, and fluorescence detectors is described. With the proposed method the samples were analysed by direct injection without a previous treatment.

For method validation, satisfactory recoveries (> 95%) and suitable repeatabilities (within day: R.S.D. (n=6=) < 3%, and between days; R.S.D. (n=6=) < 3%) were achieved.

The method was applied to the analysis of commercially available red wines.

Keywords:

Chromatographic analysis, direct injection, malvin.