The Open Gastroenterology Journal

2009, 3 : 19-22
Published online 2009 September 2. DOI: 10.2174/1874259900903010019
Publisher ID: TOGASJ-3-19

Upregulation of Intestinal NaP-IIb Co-Transporter Gene Expression by Neuropeptide Y in Human Intestinal Caco-2 Cells

Kenji Tsuchida , Maki Saito , Tsutomu Nakahara , Kenji Sakamoto and Kunio Ishii
Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences. 9-1 Shirokane 5-chome, Minato-ku, Tokyo 108-8641, Japan

ABSTRACT

The aim of the present study is to examine the effect of neuropeptide Y and estrogen on type IIb sodiumcoupled phosphate co-transporter (NaPi-IIb) gene expression and activity. Uptake studies with human intestinal cell line, Caco-2 cells, showed that treatment with neuropeptide Y and estrogen concentration-dependently increased sodiumdependent phosphate absorption. Reverse transcriptase-polymerase chain reaction analysis indicated that expression of NaPi-IIb mRNA was also increased after Y treatment with neuropeptide and estrogen. These studies show for the first time that neuropeptide Y increased intestinal sodium-dependent phosphate absorption. This increase is associated with the increase of the expression of NaPi-IIb mRNA

Keywords:

Type IIb sodium-phosphate co-transporter, SLC34A2, Caco-2 cells, Neuropeptide Y, Estrogen.