Preparation of Eucommia ulmoides Leaves Antioxidant and Its Antioxidation Activity In Vitro and In Vivo

ABSTRACT

The adsorption and desorption efficiencies on flavonoids and polyphenols from Eucommia ulmoides leaves by different resins were investigated, and the most suitable resin for preparation of Eucommia ulmoides leaves antioxidant was chosen. Then the crude extract of Eucommia ulmoides leaves was adsorbed by resin, and the resin was eluted by 20%, 40%, 60% and 80% (in volume) ethanol elution solution, and then the antioxidants were gotten. The flavonoids and polyphenols in the antioxidants were tested for content. The antioxidation activity in vitro was studied by testing the scavenging capacities on HO·, O2 -· and DPPH·. The antioxidation activity in vivo was studied by measuring the content of malondialchehyche (MDA), and superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activity in mice orally administrated by 20% and 60% ethanol elution solution sample. The result showed: the 20%, 40% and 60% ethanol elution solution had good scavenging capacities on HO·, O2 -· and DPPH·, and the antioxidation activities in vitro of the antioxidants was related to flavonoids and polyphenols, and polyphenols was the leading working material in antioxidation in vitro; 20% ethanol elution solution, of which there was more polyphenols in, showed more obvious antioxidation activities in vivo, which also meant that polyphenols was the leading working material in antioxidation in vivo.