The Open Nanoscience Journal

2008, 2 : 34-38
Published online 2008 September 10. DOI: 10.2174/1874140100802010034
Publisher ID: TONANOJ-2-34

Glucose Biosensor Based on Chitosan-Gold and Prussian Blue-Gold Nanoparticles

Juozas Kulys and Robert Stupak
Vilnius Gediminas Technical University, Sauletekio Avenue 11, LT-10223 Vilnius, Lithuania.

ABSTRACT

Amperometric glucose biosensors were constructed by codeposition of glucose oxidase (GOx) with chitosangold (chitosan-AuNP) on gold-Prussian Blue (Au-PB) nanoparticles modified glassy carbon electrodes (GCE). The high stability of the biosensors was achieved using electrochemically controllable in situ Au-PB film preparation following the film covering with GOx layer encrusted with chitosan-AuNP. The biosensors showed 95 % response time up to 10 s, sensitivity up to 9.5 µA M-1 cm-2 and linear calibration graph up to 3 mM of glucose.

Keywords:

Nanoparticle, gold, glucose.