The Open Natural Products Journal

2013, 6 : 5-11
Published online 2013 May 17. DOI: 10.2174/1874848101306010005
Publisher ID: TONPJ-6-5

Antioxidant Polyphenolic Constituents of cv. ‘Isabella’ Leaves

Severina Pacifico , Brigida D’Abrosca , Monica Scognamiglio , Marialuisa Gallicchio , Silvia Galasso , Pietro Monaco and Antonio Fiorentino
Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche (DiSTABiF), Seconda Università degli Studi di Napoli, via Vivaldi 43, 81100 Caserta, Italy.

ABSTRACT

The polyphenolic composition and antioxidant properties of the methanol leaf extract of Vitis × Labruscana cv. ‘Isabella’ has been performed by means of different spectrophotometric antioxidant methods. Intracellular ROS production was measured by cell fluorescence after loading with 2',7'-H2DCF-DA. The extract promising efficacy has laid the basis to its phytochemical characterization. Three hydroxycinnamoyl tartaric acids (1-3 ), isoquercitrin 4, and five flavonol glycuronides (5-9) were isolated from Vitis × Labruscana cv. ‘Isabella’ leaves. Chemical structures were elucidated on the basis of their spectroscopic features. Metabolites 6, 7, and 9, characterized by glucuronic or galacturonic acid methyl ester, are described from the genus Vitis for the first time. The metabolites isolated as major constituents were investigated for their DPPH radical scavenging capacity.

Keywords:

cv. ‘Isabella’, polyphenols, antioxidant activity.