Relative Bioavailability of Zinc in Yogurt Using Body Weight Gain, Femur Weight and Bone Zinc Content in Rats as Markers

ABSTRACT

Objective: some previous works have shown the importance of zinc in nutrition, and even evaluated different zinc sources for food supplementation with this micronutrient. The aim of this work was to evaluate zinc gluconate stabilized with glycine (BZ) as a zinc source for food fortification by employing yogurt as the vehicle and zinc sulfate (SZ) as the standard zinc source. Materials and methods: weaned Sprague Dawley rats were separated in 7 groups of 10 rats each, which were fed with diets according to the scheme: Group 1- 2ppm Zn; Groups 2, 3 and 4 - 9, 20 and 34 ppm Zn as SO₄Zn.7H₂O (SZ) and Groups 5, 6 and 7 - 9, 20 and 34 ppm Zn as zinc gluconate stabilized with glycine (BZ) during 21 days. Zinc sources were provided in a vehicle as yogurt, added in the final diet at 10%. Body weight gain, femur weight and femur zinc content were evaluated at the end of the treatments in order to determine zinc bioavailability, as previously described by others. In this way, body weight gain and femur weight were analyzed by a non linear regression according to equation: Y = Ymax (1-e_(-kX)) and femur zinc content was analyzed by a linear regression, all of them as a function of dietary zinc from each source. Results: relative zinc bioavailability of BZ was 0.95; 0.95 and 1.10 according to body weight gain, femur weight and femur zinc content, respectively. Conclusion: zinc gluconate stabilized with glycine may be considered as a suitable source of zinc for food fortification in a yogurt matrix.

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