Identification of Up-Regulated Low Molecular Weight Proteins in Human Adipocytes Treated with Glycoxidized Albumin

ABSTRACT

Diabetes and Obesity are strongly associated with enhanced circulating advanced glycoxidation end products (AGEs), and adipose tissue constitutes a biological driver in the metabolic syndrome characterized by abdominal obesity, dyslipidemia and insulin resistance. We determined whether AGEs issued from glycoxidized albumin could induce expression of determinant proteins in human adipocyte cell lines (SW872). Cell proteins separation by 2D gels and mass spectrometry were combined to identify up-regulated proteins in SW872 treated with AGEs. Four low molecular weight proteins appeared as more expressed in adipocytes treated with glycated albumin than with native albumin. All four proteins have determinant role in adipocyte physiology as they exert antioxidant activities (superoxide dismutase and proteasome subunit) or energy production properties (phosphoglycerate mutase and triosephosphate isomerase). Proteomic analyses of SW872 cells have never been performed before. By using proteomic analyses, our results bring new evidence of AGEs-involvement in metabolic disorders at the adipocyte level.