The Open Pathology Journal

2009, 3 : 30-32
Published online 2009 April 10. DOI: 10.2174/1874375700903010030
Publisher ID: TOPATJ-3-30

Detection of by SYBR Green Real Time PCR

Keld Andresen , Rimtas Dargis , Michael Kemp and Jens Jørgen Christensen
Department of Bacteriology, Mycology and Parasitology, Statens Serum Institute, Artillerivej 5, DK- 2300 Copenhagen S, Denmark

ABSTRACT

Development of rapid and sensitive techniques for detection of B. pseudomallei is an important aim for public health. Here we report a B. pseudomallei specific SYBR Green-based real time PCR targeting a species specific toxin gene. The assay is able to detect 1-2 genome equivalents of B. pseudomallei

Keywords:

Melioidosis, , PCR detection.