The Open Conference Proceedings Journal

2010, 1 : 98-102
Published online 2010 August 24. DOI: 10.2174/22102892010010100098
Publisher ID: TOPROCJ-1-2-98

A New Strategy for the Synthesis of Nucleosides: One-Pot Enzymatic Transformation of D-Pentoses into Nucleosides

Anatoly I. Miroshnikov , Roman S. Esipov , Tat'yana I. Muravyova , Irina D. Konstantinova , Ilja V. Fateev and Igor A. Mikhailopulo
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 220141 Minsk, Acad. Kuprevicha 5/2, Republic of Belarus.

ABSTRACT

A possibility of the one-pot synthesis of purine and pyrimidine nucleosides employing pure recombinant ribokinase, phosphopentomutase and nucleoside phosphorylases in a caskade transformation of D-pentoses into nucleosides is demonstrated. Preliminary results of this study point to reliability to develop practical methods for the preparation of a number of biologically important nucleosides.

Keywords:

Nucleosides, one-pot transformation of pentoses into nucleosides.