The Open Conference Proceedings Journal

2013, 4 : 123 -123
Published online 2013 March 01. DOI: 10.2174/2210289201304010123
Publisher ID: TOPROCJ-4-4-123

Antibacterial and Antihypertensive Activity OF Callus from Muskmelon (Cucumis Melo L.)

Nurul Ashikin Binti Abdul Karim , Natanamurugaraj Govindan , Tan Suat Hian and Mashitah Mohd Yusoff
Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, 26300 Gambang, Kuantan, Pahang, Malaysia.

ABSTRACT

The callus of Cucumis melo L. (Muskmelon var. glamour) was studied to identify the polyphenols that have possible antihypertensive and antibacterial activity. The callus was grinded and extracted by solvent extraction. Total polyphenols were quantified using modified method of Folin-Ciocalteu and total flavonoid was quantified by modified method of Aluminium chloride complex formation. The antibacterial activity was tested against Gram-positive and Gram-negative human pathogenic bacteria using disk diffusion method and the Minimum Inhibitory Concentration (MIC) was also determined. The antihypertensive property was tested with ACE Kit. The phytohormone combination for callus induction was better when the concentration of auxin is higher than the concentration of cytokinin. Different phytohormone combination treated in media for callus induction gave different effect to the production of polyphenols of plant. The callus’s polyphenols analyzed in GC/MS indicated some polyphenols compounds that possessed antihypertensive property and involved against pathogenic bacteria.

Keywords:

Cucumis melo , callus culture , Antibacterial , Antihypertensive activity .