The Open Conference Proceedings Journal

2013, 4 : 52-52
Published online 2013 March 1. DOI: 10.2174/2210289201304010052
Publisher ID: TOPROCJ-4-4-52

Antiinflammatory, Anticholinesterase and Antioxidant Acitivites of Scopoletin Isolated from Canarium Sp. (Burseraceae Kunth)

R. Mogana , Khoo Teng Jin and Christophe Wiart
School of Pharmacy, Faculty of Science, University of Nottingham (Malaysia Campus), Jln Broga, Semenyih, 43500, Selangor Darul Ehsan, Malaysia.

ABSTRACT

Bioassay guided fractionation of an ethanol extract of leaves of Canarium sp. (Burseraceae Kunth.) led to the isolation of scopoletin. The structure of this coumarin was elucidated based on spectroscopic methods including nuclear magnetic resonance (NMR-1D and 2D), and mass spectrometry. Scopoletin inhibited the enzymatic activity of 5-lipooxygenase and acetylcholinesterase with an IC50 equal to 1.76 μM and 0.27mM, respectively and confronted oxidation in the ABTS, DPPH, FRAP and β-carotene bleaching assay with EC50 values equal to 5.62μM±0.03, 0.19mM±0.01, 0.25mM±0.03 and 0.65mM±0.07 respectively. Scopoletin being anti-inflammatory, acetylcholinesterase inhibitor and antioxidant might be of value for the treatment of neurodegenerative diseases including Alzheimer’ diseases, Parkinson’s disease and Amyotrophic Lateral Sclerosis. Further in vivo studies are warranted.

Keywords:

Canarium sp., antiinflammatory, antioxidant, coumarin, scopoletin.