The Open Conference Proceedings Journal

2013, 4 : 56-56
Published online 2013 March 1. DOI: 10.2174/2210289201304010056
Publisher ID: TOPROCJ-4-4-56

Antioxidant Activity of Sala ( Linn) Plant Extract

Sontaya Sookying , Dumrongsak Pekthong , AM Oo-puthinan , Jie Xing , Zhaoqi Zhan and Kornkanok Ingkaninan
Faculty of Pharmacy, Muhammadiyah University of Surakarta, Jl. Achmad Yani Tromol Pos 1, Pabelan Kartasura, Surakarta.

ABSTRACT

To investigate the antioxidant activity of Sala (Cynometra ramiflora Linn) medicinal plant extract were successively extracted with methanol and fractionation with the chromatography method to get nonpolar, semipolar and polar fraction. The leaves, fruits and stem barks of Sala had been extracted with methanol and screened for antioxidant activity using DPPH assay. The fruit extract and polar fraction of Sala leaves extracts showed low antioxidant activity, with the IC50 values respectively, 425.10 ppm and 183.57 ppm. The methanolic extract, semipolar fraction of leaves extracts, nonpolar and semipolar fraction of stem bark extract showed a potential antioxidant activity, in that order 54.44, 91.20, 79.64 and 79.59 ppm. The stem bark methanol extract of Cynometra ramiflora Linn showed the highest activity (IC50 41.90 ppm). The result showed that prospective antioxidant of leaves and stem bark extract was higher than the fractions. Based on the IC50 values the extract, semipolar and nonpolar fraction of stem bark can be developed as antioxidant agent.

Keywords:

Antioxidant, Cynometra ramnifolia Linn, mass spectrometry, and DPPH assay.