Amplification of Brugia malayi DNA using Hha1 Primer as a Tool

ABSTRACT

Lymphatic filariasis, a neglected parasitic disease caused by tissue dwelling human filarial nematodes such as Wuchereria bancrofti, Brugia malayi and B. timori is considered to be a major complication for the socio-economic development in developing countries. A number of inflammatory responses are associated with the diseases such as adeno-lymphangitis, lymphoedema, hydrocele and elephantiasis. The present study is directed towards the identification and amplification of Hha 1 gene for diagnosis of B. malayi. The specific primer Hha1 specific to Brugia malayi was used for detecting the parasites and was found to give optimum yield in the positive control samples. The results were confirmed from the amplified fragment having size of 322 bp of B. malayi. Using this primer as a diagnostic tool for the detection of filariasis might be the most promising aspect of the study and offers scope for detection of both the parasites even at low levels of infection.

Keywords: