The Open Conference Proceedings Journal
2016, 7 : 104-113Published online 2016 May 13. DOI: 10.2174/2210289201607010104
Publisher ID: TOPROCJ-7-1-104
RESEARCH ARTICLE
Antibacterial Activity and Preliminary Phytochemical Screening of Endophytic Fugal Extract of
2 Department of Kayachikitsa, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India
3 Department of Dravyaguna, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India
* Address correspondence to this author at the Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India; Tel: +91-9415389046; Email: singhsk71@yahoo.com
ABSTRACT
Endophytic fungi isolated from Rauvolfia serpentina, a well known Indian medicinal plant, is used in Ayurveda for treatment of many diseases. Isolated endophytes were screened for their antibacterial activity against pathogenic bacteria. Twenty fungal isolates were recovered from different parts of the host plant and they were characterized for their morphological features through Scanning Electron Microscopy (SEM) and on the basis of observations they were grouped in eight genus as Fusarium sp., Phomopsis sp., Colletotrichum sp., Cladosporium sp., Aspergillus sp., Xylaria sp., Alterneria sp. and Gleomastix sp. The secret of the fungal endophytes of this medicinal plant was revealed by the evaluation of the extract against the target bacteria. The extracts of four fungal isolates Colletotrichum sp. (Rs-R5), Fusarium sp.(Rs-R1), (Rs-R7) and Cladosporium sp.(Rs-S4) among twenty isolates were found effective against human pathogenic bacterial strains E. coli (ATCC 25922), Gram negative bacteria and S. aureus (ATCC 25323), Gram positive bacteria. Ethyl acetate extract of active fungal isolate (Colletotrichum sp; Rs-R 5) was most effective than other extract, with maximum inhibition zone 16 mm and 14 mm and minimum MIC 25µg/ml and 36.5µg/ml against E. coli and S. aureus respectively.