The Open Proteomics Journal

2012, 5 : 22-28
Published online 2012 October 19. DOI: 10.2174/1875039701205010022
Publisher ID: TOPROTJ-5-22

PI-Based Separation of Tissue Proteins Realized with the Use of Two-Dimensional Gel Electrophoresis or Alternatively Capillary Isoelectric Focusing

Ilona Oledzka , Kamil Wlodarski , Piotr Kowalski , Jolanta Grzenkowicz-Wydra , Jolanta Paradziej-Lukowicz and Tomasz Baczek
Department of Pharmaceuti-cal Chemistry, Medical University of Gdansk, Hallera 107, 80-416 Gdansk, Poland.

ABSTRACT

A two-dimensional gel electrophoresis (2D-GE) and capillary isoelectric focusing (cIEF) were performed sub-sequently to analyze protein mixtures from laboratory animal tissues (liver, muscle, and mammary gland). Both tech-niques were compared and evaluated. It was found that cIEF can be a valuable alternative in comparison to 2D-GE. Espe-cially considering the goals associated with the determination of far the separated proteins, cIEF possesses perspective analytical advantages.

Keywords:

Two-dimensional gel electrophoresis (2D-GE), Capillary isoelectric focusing (cIEF), Animal tissue.