The Open Proteomics Journal

2014, 7 : 1-9
Published online 2014 February 07. DOI: 10.2174/1875039701407010001
Publisher ID: TOPROTJ-7-1

Lipid MALDI MS Profiles of Gastric Cancer

Sun Young Kwon , Seung Ho Choi , Young Soo Park , Do Youn Park , Young Iee Park , Ilseon Hwang , Min Hee Ryu , Chae Hwa Kwon , Jeong Hwa Lee , Geul Bang , Kwang Pyo Kim and Hark Kyun Kim
the Biomolecular Function Research Branch, Research Institute, National Cancer Center, 323 Ilsanro, Ilsan, Goyang, Gyeonggi, 410-769, Republic of Korea;

ABSTRACT

Tissue matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)may identify lipids differentially expressed between cancer and adjacent normal tissue. To identify lipidomic profiles for gastric cancer, 24 gastric cancerswere profiled for lipid by the histology-directed, tissue MALDI MS technology. Lipid profiles differed between gastric cancer and adjacent normal tissue samples. At P<0.05, median class prediction accuracy in 100 random training-to-test partitions was 83.3% (5/6) for all classifiers tested. A peak at m/z 741.6 (sphingomyelin 34:1 K) was overexpressed, and a peak at m/z 782.6 (phosphatidylcholine 34:1 Na) was underexpressed in gastric cancers compared with normal tissue. Thus, lipid MALDI MS analysis may capture a global alteration in lipid profile of gastric cancer tissue, distinguishing cancerous epithelium from normal epithelium

Keywords:

Gastric cancer, lipid, MALDI, profile.