The Open Plant Science Journal

2017, 10 : 100-109
Published online 2017 September 14. DOI: 10.2174/1874294701710010100
Publisher ID: TOPSJ-10-100

RESEARCH ARTICLE
 Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry

Hongxia Li1,2 , Natasha Trzaskalski3, * and R.J. Neil Emery1

* Address correspondence to this author at the Department of Biology, Trent University, Peterborough, ON, K9J 7B8, Canada, Tel: 705-748-1011 7601, Fax: 705-748-1003; E-mail: ntrzaskalski@trentu.ca

ABSTRACT

Objective:

A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.

Materials:

The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.

Method:

Five different purification strategies have been tested for the purification and enrichment of BRs.

Conclusion:

This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.

Keywords:

Brassinosteroids, HPLC-ESI-MS/MS, Castasterone, Brassinolide, 24-Epibrassinolide, 24-Epicastasterone.