The Open Plant Science Journal
2009, 3 : 35-39Published online 2009 July 2. DOI: 10.2174/1874294700903010035
Publisher ID: TOPSJ-3-35
In Vitro Propagation of Arundinaria callosa Munro- an Edible Bamboo from Nodal Explants of Mature Plants
ABSTRACT
A procedure for in vitro propagation of Arundinaria callosa Munro has been developed. The method allows bud-break from nodal segments containing single axillary bud on Murashige and Skoog (1962) medium supplemented with different concentrations of 6- benzylaminopurine (BAP). The early bud-break was obtained in 8.9-13.3 μM BAP, within 8-15 days. The position of the node on the culm of lateral branches also affected bud-break percentage and multiplication, mid-culm nodes are the most suitable. The optimal concentration of 13.3 μM BAP is found significant for shoot multiplication. Addition of 1.0 μM 3- indolebutyric acid (IBA) enhances the shoot multiplication rate. In vitro rooting was induced when 15 μM IBA was incorporated for three subcultures in the shoot proliferation medium, was transferred to strength MS containing 25μM IBA and 0.05 μM BAP, and finally withdrawn from the rooting medium. The regenerants were successfully transplanted into a soil mixture for acclimatization before field planting.