The Open Stem Cell Journal

2009, 1 : 1-9
Published online 2009 January 21. DOI: 10.2174/1876893800901010001
Publisher ID: TOSCJ-1-1

Comparative Profiling of mRNA and microRNA Expression in Human Mesenchymal Stem Cells Derived from Adult Adipose and Lipoma Tissues

Li-Pin Kao , Sung-Liang Yu , Sher Singh , Kai-Hung Wang , An-Pei Kao and Steven Shoei-Lung Li
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

ABSTRACT

Human mesenchymal stem cells have been isolated from adult adipose tissue and lipoma that is a benign neoplasm of normal fat cells. The lipoma-derived LM6 cells exhibited a higher cumulative population doubling levels as compared with the adipose-derived AM3 cells. The expression profiles of both mRNAs and microRNAs (miRNAs) from AM3 and LM6 cells were found to exhibit considerable similarities except that miRNAs miR-99a and miR-152 were abundantly expressed in AM3, but absent in LM6 cells. The abundantly differentially expressed genes HAS2, VNN1, SLC16A6 and COL11A1 in LM6 cells were shown to be targets of miRNAs miR-99a and/or miR-152. These highly upregulated miRNA target genes, as well as several other abundantly differentially expressed genes such as sushi domain containing 2, keratin associated proteins and tumor necrosis factor family, may explain a higher proliferation potential in LM6 cells compared with AM3 cells

Keywords:

Human adipose, mesenchymal stem cell, profiling, mRNAs, miRNAs.