The Open Spectroscopy Journal

2009, 3 : 21-25
Published online 2009 February 13. DOI: 10.2174/1874383800903010021
Publisher ID: TOSPECJ-3-21

Differentiation Between Pure Cultures of Streptococcus pyogenes and Pseudomonas aeruginosa by FT-ICR-MS Volatile Analysis

Jan E. Szulejko , Sally K. Hall , Mark Jackson and Touradj Solouki
Chemistry Department, 5706 Aubert Hall and 2Student Health Services, Cutler Health Center, University of Maine, Orono, ME 04469-5706, USA.

ABSTRACT

Two common bacteria, Streptococcus pyogenes and Pseudomonas aeruginosa, were differentiated based on their volatile metabolic waste products. The bacteria were cultured in a closed system and the headspace above the culture medium were collected, preconcentrated, and analyzed using a gas chromatography Fourier transform ion cyclotron resonance mass spectrometer (GC/FT-ICR MS).