The Open Systems Biology Journal

2010, 3 : 29-31
Published online 2010 May 6. DOI: 10.2174/1876392801003010029
Publisher ID: TOSYSBJ-3-29

RESEARCH ARTICLE
 Quartz Crystal Microbalance Immunoassay of Hepatitis B Surface Antigen Using Magnetic CoFeO/CuO Composite Nanoparticles as Immunosensing Probe

Feng Tian1 and Dianping Tang, *,2
1School of Fine Arts, Chongqing Three Gorges University, Wanzhou, Chongqing 404000, P.R. China
2College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, P.R. China

*Address correspondence to this author at the College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, 350108, P.R. China; Tel: +86 591 2286 6125; Fax: +86 591 2286 6135; E-mail: dianping.tang@hotmail.com

ABSTRACT

This study describes a new strategy toward the development of advanced immunosensors based on chemically functionalized magnetic-core/porous-shell CoFe2O4/Cu2O composite nanoparticles, and the preparation, characterization, and measurement of relevant properties of the immunosensor useful for the detection of hepatitis B surface antigen (HBsAg) in clinical immunoassay. The immunosensor based on the combination of a magnetic nanocore and a Cu2O shell shows good adsorption properties for the attachment of hepatitis B surface antibody (HBsAb) selective to HBsAg. The core-shell nanostructure presents good magnetic properties to facilitate and modulate the way while it was integrated into a magnetic interface. Under optimal conditions, the resulting composite presents good frequency response for the detection of HBsAg, and allows detection of HBsAg at concentration as low as 0.5 ng/mL. Importantly, the proposed methodology could be extended to the detection of other antigens or biocompounds.

Keywords:

Hepatitis B surface antigen, immunosensor, nanoparticles, quartz crystal microbalance.