The Open Tissue Engineering and Regenerative Medicine Journal

2008, 1 : 8-13
Published online 2008 June 13. DOI: 10.2174/1875043500801010008
Publisher ID: TOTERMJ-1-8

Sequential Use of Fibroblastic Growth Factor 2 and Transforming Growth Factor β1 in Cartilage Tissue Engineering Using Canine Chondrocytes

Wanda J. Gordon-Evans , Michael J. Yaeger , M. Heather Greenlee and Michael G. Conzemius
Department of Veterinary Clinical Medicine, University of Illinois, Urbana-Champaign. Formerly Department of Biomedical Sciences Iowa State University, USA.

ABSTRACT

This study investigated the effect of fibroblast growth factor 2 in monolayer followed by transforming growth factor β1 in 3-dimensional culture on chondrocytic matrix production. Under the culture conditions presented, FGF2 is mitogenic but not chondrogenic. In addition, TGFβ1 stimulation produced predominantly type I collagen.