The Open Tissue Engineering and Regenerative Medicine Journal

2009, 2 : 14-19
Published online 2009 May 7. DOI: 10.2174/1875043500902010014
Publisher ID: TOTERMJ-2-14

Immobilisation of Cells in Biocompatible Films to Cell Therapy

Valle Eva. M. Martín Del , Herrero Edgar P. , Martins Dina A. O. and Galán Miguel. A.
Department of Chemical Engineering, University of Salamanca, P/Los Caídos S/N, 37008, Salamanca, Spain.

ABSTRACT

In this work a new system for immobilisation of cells was developed based on biocompatible alginate bariumfilms. Films are prepared to allow better control over film thickness and produce better film uniformity, avoiding roughness over film surface that can produce fibrosis and rejection after transplantation.

In this study monocyte, mononuclear and fibroblast cell lines were immobilised in micrometric (≈300 microns of thickness) barium alginate films. The system was able to maintain the cell viability of all the three types of cells for at least two weeks after immobilisation, but cell proliferation was only verified for the monocyte and fibroblast cell lines.

Keywords:

Immobilisation, alginate barium membranes, cell therapy.