The Open Tropical Medicine Journal

2011, 4 : 39-43
Published online 2011 December 16. DOI: 10.2174/1874315301104010039
Publisher ID: TOTMJ-4-39

Brain Damages by Tripanossoma cruzi Increased Blood Brain Barrier Permeability in Holtzman Rats: Blood Brain Barrier Permeability in Chagas' Disease

Joao Roberto da Mata , Fabiana Ribeiro da Mata , Hisao Nishijo and Tales Alexandre Aversi-Ferreira
System Emotional Science, Graduate School of Medicine and Pharmaceutical Sciences University of Toyama 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT

The T. cruzi strains are a complex of multi-clonal populations that affects the brain with more severity in children or young animals, suggesting that functional immaturity of the blood brain barrier (BBB) might be involved in T. cruzi infection in the brain. This study investigated relationships between BBB permeability and T. cruzi infection in the rat brain. The animals were inoculated with one of 3 strains of T. cruzi at 30 days of age, and BBB permeability was increased by subcutaneous kainic acid injection in half of the infected animals. The animals were sacrificed at 13 days of infection. The brain sections were stained with hematoxylin and eosin. The all brains of the infected animals with and without kainic acid showed both glial nodules and perivascular infiltrates due to T. cruzi infection. Number of these glial nodules was counted in each rat, and statistical comparison indicated that kainic acid-treatment significantly increased mean numbers of glial nodules in all of the rats infected with the 3 strains. The results indicated that the increased BBB permeability was one of the factors for facilitation of the entry of T. cruzi into the brain.