The Open Virology Journal
2013, 7 : 91-95Published online 2013 October 18 . DOI: 10.2174/1874357920130927004
Publisher ID: TOVJ-7-91
RESEARCH ARTICLE
Detection and Genotyping of Human Papillomavirus DNA in Formalin-Fixed Paraffin-Embedded Specimens with the HPV Direct Flow CHIP System
2 Pathology Department, Elda General Hospital. Carretera Elda-Sax, La Torreta s/n, 03600, Elda, Alicante, Spain
3 Pathology Department, Ruber Clinic, Calle de Juan Bravo, 49, 28006, Madrid, Spain
* Address correspondence to this author at the R&D Department, Master Diagnóstica, Avenida del Conocimiento 100, Parque Tecnológico de Ciencias de la Salud, 18016, Granada, Spain; Tel: +34 958 271 449; Fax: +34 958 271 434; E-mail: asuncion.olmo@vitroweb.com
ABSTRACT
The novel HPV Direct Flow CHIP commercial system for Human Papillomavirus (HPV) genotyping is based on rapid PCR and automatic reverse dot blot hybridization to genotype-specific probes, allowing the detection of 36 HPV genotypes. This study examined the performance of HPV Direct Flow CHIP in formalin-fixed paraffin-embedded (FFPE) samples (n= 99). Each sample was analyzed both by Direct PCR, using crude cell extracts without DNA purification, and by conventional PCR, using purified DNA. Pair-wise analysis of the results demonstrated strong concordance between the results obtained with the two protocols, although a slightly higher rate of multiple infections was detected by conventional PCR. In summary, HPV Direct Flow CHIP achieves effective HPV detection from FFPE samples with both Direct PCR and Conventional PCR protocols.