The Open Veterinary Science Journal

2008, 2 : 11-15
Published online 2008 February 14. DOI: 10.2174/1874318808002010011
Publisher ID: TOVSJ-2-11

Tumorcidal Effect of Recombinant Newcastle Disease Virus

Katsuro Hagiwara , Tsuyoshi Kadosawa and Takaaki Nakaya
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501 Japan.

ABSTRACT

We have generated GFP expressing recombinant Newcastle Disease Virus (rNDV-GFP) based on a live vaccine strain which contains a less pathogenic cleavage site of F protein leading to restricted viral growth in vivo. We evaluated the rNDV have an effect of oncolytic activity to B16 melanoma cell. The rNDV-GFP infected efficiency to B16 cells and induced cell death. Apoptosis-related genes, Bax and Caspase 3, were elevated in the infected cells. In order to evaluate the tumorcidal effect of rNDV on canine tumor cells, two canine tumors such as highly metastasizing canine osteosarcoma (HMPOS) and transitional cell carcinoma (TCC) were infected with rNDV. The rNDV induced cell death to canine tumor cells within 48 hours post infection. These results suggest that rNDV-GFP have a tumorcidal activity to B16 mouse melanoma and canine tumors in vitro.

Keywords:

NDV, oncolytic, melanoma, HMPOS, TCC.