The Open Veterinary Science Journal

2012, 6 : 23-29
Published online 2012 October 5. DOI: 10.2174/1874318801206010023
Publisher ID: TOVSJ-6-23

The Effects of Pre-Analytical Processing and Storage on Bovine Blood D- and L-Lactate Concentrations

Binbing Ling , Jennifer Wright , Kimberley Buker , Jane Alcorn , Gordon Zello and Katharina Lohmann
Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada.

ABSTRACT

To investigate processing- and storage-dependent changes in D- and L-lactate concentration, blood samples from eleven healthy Holstein calves were spiked with 3 mM D-lactic acid and 3 mM L-lactic acid immediately following collection (time 0) or left untreated for comparison. Serum and plasma, respectively, were separated 0.5 hours following collection or left in contact with blood cells, stored at 4°C and analyzed for D- and L-lactate concentration using enzymatic assays at 1, 2, 4, 8, 12, 24, and 48 hours. Concentrations were compared to the 1 hour sample. D- and L-Lactate concentrations in all separated samples were stable for up to 48 hours. When left in contact with cells, L-lactate concentration in untreated and spiked serum and in spiked plasma, D-Lactate concentration in untreated serum, and total lactate concentration in untreated serum increased significantly by 48 hours.

Keywords:

Specimen Collection, centrifugation, lactate isomers.