TOBIOJ The Open Biology Journal Open Bio. J. 1874-1967 Bentham Open TOBIOJ-2-27 10.2174/1874196700902010027 Biology Indexing Tools for Indian Citrus Ringspot Virus (ICRSV) Sharma Sanjeev * 1 Singh Balwinder 2 Nagpal Avinash 3 S. Virk G. 3 A. Zaidi A. 4 Lyallpur Khalsa College, Jalandhar-144001, India Khalsa College, Amritsar-143001, India Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India Molecular Plant Virology Laboratory, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur 176061 (H.P.), India Address correspondence to this author at the Department of Botany, Lyallpur Khalsa College, Jalandhar-144001, India; E-mail: sanbot@rediffmail.com 01 4 2009 2009 2 27 31 11 11 2008 12 12 2009 27 01 2009 © 2009 Singh et al. 2009 Balwinder Singh

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Indian citrus ringspot virus (ICRSV) is known to cause serious disease problem in Kinnow (Citrus nobilis Lour 􀀁 C. deliciosa Tenora). This paper reports the various methods viz. Bioassay, ELISA and RT-PCR for indexing of ICRSV. Bioassay was performed on Chenopodium amaranticolor, Cucumis sativus, Nicotiana glutinosa, N. tabacum, Petunia hybrida and Phaseolus vulgaris. However necrotic local lesions were observed only in case of Chenopodium amaranticolor and Phaseolus vulgaris. Infected trees were also found positive by indirect ELISA. RT-PCR of the infected plants showed an amplification of 539 bp fragment corresponding to coat protein gene and gene for nucleic acid binding protein.

 Keywords Kinnow ICRSV Bioassay ELISA RT-PCR