The Open Clinical Biochemistry Journal

2018, 8 : 1-6
Published online 2018 April 30. DOI: 10.2174/2588778501808010001
Publisher ID: TOCCHEMJ-8-1

RESEARCH ARTICLE
The Effect of Lycopene Treatment on Oxidative DNA Damage of Experimental Diabetic Rats

F. Karahan1 , S. Dede1, * and E. Ceylan2
1 Biochemistry Department, Veterinary Medicine School, Van Yuzuncu Yil University, Van, , .
2 Internal Disease Department, Veterinary Medicine School, , ,

* Address correspondence to this author at the Biochemistry Department, Van Yuzuncu Yil University, Veterinary Medicine School, Van, 65080, Turkey; Tel: +904322251027/1520; Fax: +904322251127; E-mail: sdede@yyu.edu.tr

ABSTRACT

Objective:

Lycopene is a carotenoid with anti-inflammatory and antioxidant properties. The aim of this study was to determine the effects of lycopene on oxidative DNA damage levels in experimental diabetic rats.

Subjects and Methods:

Four experimental groups, each consisting of 7 rats, were prepared as Controls, Diabetes (D), Lycopene-treated diabetes (DL) and Lycopene (L). STZ (45 mg/kg) was administered to the diabetic groups intraperitoneally in a single dose. Lycopene was administered to the L and DL groups (10 mg lycopene/kg/day). The test procedure continued for four weeks. To understand the occurrence of diabetic conditions, serum glucose and HbA1c% in the whole blood were determined. The 8-OHdG levels, a marker of oxidative DNA damage, were determined in the blood serum.

Results:

Blood glucose and HbA1c% were higher in the DL group than in the control group and L group (p <0.05) and lower in the D group (p <0.05). 8-OHdG levels were higher in D group than the other groups (p <0.05) while 8-OHdG levels in DL group were lower than D group (p <0.05) and approximated to the control group.

Conclusion:

It can be suggested that lycopene may be described as a protective agent to prevent oxidative DNA damage originated from diabetes.

Keywords:

Oxidative DNA damage, Diabetes, Lycopene, Rats, STZ, Mutagenesis.