The Open Fish Science Journal

2008, 1 : 1-7
Published online 2008 March 10. DOI: 10.2174/1874401X00801010001
Publisher ID: TOFISHSJ-1-1

Partial cDNA Sequence Analysis of Myosin Va from Rainbow Trout (Oncorhynchus mykiss) and Its Relationship to Myosin V Isoforms from Other Vertebrates

Kátia Gisele Oliveira Rancura , Cesar Martins , Michelli Rivero Montaño , Robson Francisco Carvalho and Alexandre Azevedo
Cesar Martins, Departamento de Morfologia, Instituto de Biociências, UNESP – Universidade Estadual Paulista, 18618-000, Botucatu, SP, Brazil.

ABSTRACT

Partial cDNA sequences of myosin V from rainbow trout Oncorhynchus mykiss were analyzed and showed high similarity to MVa from other vertebrates. Phylogenetic analysis has shown that events resulting in the formation of paralogous copies of myosin Va, Vb, and Vc occurred before the divergence of vertebrates into different classes. Expression analysis of myosin Va, Vb, and Vc in different O. mykiss tissues revealed MVa exclusively expressed in hypophysis and brain whereas Vb and Vc were expressed in practically all tissues analyzed. The nucleotide sequence for myosin V was explored in a fish species for the first time and these results represent an important start in understanding the organization, evolution, and expression of myosins in early vertebrates. The data presented here represent contributions to the knowledge of rainbow trout genome. A better understanding of this economically important species could assist in development of improved strains of this fish for aquaculture.

Keywords:

Nucleotide sequence, Amino acid sequence, Evolution.